Protein crystallisation of NikR domain using hanging drop experiment

Description

Should be composed of 5 sections: Introduction, Materials and Methods, Results, Discussion and conclusion. Max 1500 words total. must be concise. Introduction: Brief introduction to your protein target (NikR) that informs the reader about the importance of the protein. state the goals of your experiment (pretending that the structure was not known) Material and methods: state briefly the experimental procedures. Summarise which solutions were obtained, which ones were prepared and how the crystallisation experiments were set-up. describe in your own words any deviations or modifications from the instructions and identify any problems you may have encountered (none). Describe how to set this up for a diffraction experiment using Diamond, Oxford. (waiting on a file to be uploaded by lecturer on this and its results so have elected 1300 words for now but will become 1500 with the diffraction work). Results, discussion: should be presented in the form of a table or figure and summarised in a paragraph. Each table should have a table heading, each figure should have a figure legend as would be found in a research paper. Paragraph summarising most important findings. Summarise key results and suggest additional experiments that could have improved the outcome of your crystallisation experiments (ie less air bubbles in protein drop, better seal with the grease, try have anti-dust environment as some dust particles were seen under the stereomicroscopes, etc)